Journal: The Science of the Total Environment

Article Title: Sampling methods and assays applied in SARS-CoV-2 exposure assessment

doi: 10.1016/j.scitotenv.2021.145903

Figure Lengend Snippet: Data obtained from the chosen articles.

Article Snippet: , 34. SARS-CoV-2 RNA contamination on surfaces of a COVID-19 ward in a hospital of Northern Italy: what risk of transmission? , Italy , No , Surface samples from ward in University Hospital of Ferrara , Sampling performed with sterile rayon swabs pre-moistened in sterile phosphate-buffered solution , Viral RNA extraction with Patho Gene-spin Extraction kit (Generon) RT-qPCR targeted the RNA-dependent RNA polymerase ( RdRp ) gene (Generon), and the orf1ab , spike ( S ), and nucleocapsid ( N ) genes (ThermoFisher) , • SARS-CoV-2 was only detected in 3 samples of two floors and one-bathroom sink. • Reported to persist for a longer duration on surfaces under controlled laboratory conditions. , ( ) .

Techniques: Sampling, Lysis, RNA Extraction, Environmental Monitoring, Virus, Multiplex Assay, Northern Blot, Marker, RNA Detection, Isolation, Membrane, Control, Environmental Sampling, Amplification, Transmission Assay, Aerosol, Diagnostic Assay, Infection, Sterility, Real-time Polymerase Chain Reaction, Nested PCR, Reverse Transcription, Extraction, Purification, Digital PCR, Preserving, Quantitative RT-PCR, cDNA Synthesis, Magnetic Beads, Incubation, Modification, One Step RT-PCR, Cell Culture, Sequencing

Journal: Communications Biology

Article Title: DIAPH1 mediates progression of atherosclerosis and regulates hepatic lipid metabolism in mice

doi: 10.1038/s42003-023-04643-2

Figure Lengend Snippet: Ldlr − / − and Ldlr − / − Diaph1 − / − male mice were fed Western Diet (WD) for 16 weeks. a Representative images of en face Oil Red O staining of aortas. Quantification of plaque area as percentage of Oil Red O-stained area over total aortic surface area is shown. b – e Representative images of aortic arch sections are shown and quantified for the following: b H&E; c Oil Red O; d CD68; and e , Picrosirius Red. In d , the secondary antibody-alone control is shown. Scale bar: 250 µm. The mean ± SEM is reported. The number of independent mice/group is indicated in the figure as individual data points. Statistical analyses regarding testing for the normality of data followed by appropriate statistical analyses were described in Materials and Methods. P values were determined by unpaired T-test.

Article Snippet: Sections were incubated with propylene glycol for 2 min, then incubated with pre-heated Oil Red O staining solution (American MasterTech, STOROPT) at 60 °C for an additional 8 min.

Techniques: Western Blot, Staining

Journal: Communications Biology

Article Title: DIAPH1 mediates progression of atherosclerosis and regulates hepatic lipid metabolism in mice

doi: 10.1038/s42003-023-04643-2

Figure Lengend Snippet: Ldlr − / − and Ldlr − / − Diaph1 −/− male mice were fed WD for 16 weeks. a Representative immunofluorescence staining and quantification of DIAPH1 in the liver of Ldlr −/− and Ldlr −/− Diaph1 −/− male mice. b Representative images of H&E and Oil Red O staining in liver and quantification is shown. c Quantification of free cholesterol content in liver. d Quantification of total cholesterol content in liver. e Quantification of total liver triglycerides. f Representative images of Picrosirius Red staining in liver and quantification is shown. g Quantification of whole liver weight. The mean ± SEM is reported. The number of independent mice/group is indicated in the figure as individual data points. In a the secondary antibody–alone control is shown. Scale bars: 250 µm, and inset boxes: 50 µm. Statistical analyses regarding testing for the normality of data followed by appropriate statistical analyses were described in Materials and Methods. P- values were determined by unpaired T-test or Wilcoxon rank-sum test depending on if the data passed the Shapiro-Wilk normality test.

Article Snippet: Sections were incubated with propylene glycol for 2 min, then incubated with pre-heated Oil Red O staining solution (American MasterTech, STOROPT) at 60 °C for an additional 8 min.

Techniques: Immunofluorescence, Staining

Journal: Cell Stem Cell

Article Title: Mapping the Global Chromatin Connectivity Network for Sox2 Function in Neural Stem Cell Maintenance

doi: 10.1016/j.stem.2019.02.004

Figure Lengend Snippet:

Article Snippet: After pelleting and washing once with 1 mL 1 × CutSmart buffer, the nuclei were suspended in 500 μL A tailing solution composed of 50 μL 10 × CutSmart buffer, 10 μL BSA (cat#B9000S, NEB), 10 μL 10 mM dATP (cat#N0440S, NEB), 10 μL Klenow (3′-5′ exo-) (cat#M0202L, NEB), and 420 μL H2O and incubated at RT for 1 hr.

Techniques: Plasmid Preparation, Expressing, Recombinant, Sample Prep, Ligation, TA Cloning, Cell Culture, Amplification, Software